Human CGRP(Calcitonin Gene Related Peptide) ELISA Kit

 

Human CGRP(Calcitonin Gene Related Peptide) ELISA Kit

Size

96T

Catalog no.

ELK1037

Price

526 EUR

Buy at gentaur.com
Assay length

2h

Standard

1000pg/mL

Sensitivity

5.25pg/mL

Detection range

12.35-1000pg/mL

Assay Type

Competitive Inhibition

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Infection immunity;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;Dermatology;

Alternative Names

CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample.