General BSA(Bovine Serum Albumin) ELISA Kit

2h

Sera

serum

30000ng/mL

161.2ng/mL

Immune molecule;

370.4-30000ng/mL

Competitive Inhibition

Bovine Albumin; Fraction V

Serum should be stored at +5°C

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Bovine Serum Albumin (BSA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Bovine Serum Albumin (BSA) and unlabeled Bovine Serum Albumin (BSA) (Standards or samples) with the pre-coated antibody specific to Bovine Serum Albumin (BSA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Bovine Serum Albumin (BSA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Bovine Serum Albumin (BSA) in the sample.