Human ALB(Albumin) ELISA Kit

 

Human ALB(Albumin) ELISA Kit

Size

96T

Catalog no.

ELK1071

Price

526 EUR

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Assay length

2h

Sensitivity

89.6ng/mL

Standard

20000ng/mL

Alternative Names

Serum albumin

Detection range

246.9-20000ng/mL

Assay Type

Competitive Inhibition

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Albumin (ALB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Albumin (ALB) and unlabeled Albumin (ALB) (Standards or samples) with the pre-coated antibody specific to Albumin (ALB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Albumin (ALB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Albumin (ALB) in the sample.