Human b2M(Beta-2-Microglobulin) ELISA Kit

 

Human b2M(Beta-2-Microglobulin) ELISA Kit

Size

96T

Catalog no.

ELK1200

Price

526 EUR

Buy at gentaur.com
Assay length

3h

Standard

25ng/mL

Assay Type

Sandwich

Sensitivity

0.16ng/mL

Detection range

0.39-25ng/mL

Alternative Names

Beta 2M,BMG; B2MG

Research Area

Infection immunity;Kidney biomarker;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (β2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-2-Microglobulin (β2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (β2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin (β2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.