General ConA(Concanavalin A) ELISA Kit

3h

Con-A

10ng/mL

Sandwich

0.057ng/mL

0.156-10ng/mL

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Concanavalin A (ConA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Concanavalin A (ConA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Concanavalin A (ConA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Concanavalin A (ConA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.