Rat IgM(Immunoglobulin M) ELISA Kit

 

Rat IgM(Immunoglobulin M) ELISA Kit

Size

96T

Catalog no.

ELK1392

Price

526 EUR

Buy at gentaur.com
Assay length

2h

Additional isotype

IgM

Sensitivity

97.3ng/mL

Standard

20000ng/mL

Detection range

246.9-20000ng/mL

Latin name

Rattus norvegicus

Assay Type

Competitive Inhibition

Research Area

Infection immunity;Immune molecule;Hematology;

Alternative Names

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin M (IgM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Immunoglobulin M (IgM) and unlabeled Immunoglobulin M (IgM) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin M (IgM). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample.