Human AQP1(Aquaporin 1, Colton Blood Group) ELISA Kit

 

Human AQP1(Aquaporin 1, Colton Blood Group) ELISA Kit

Size

96T

Catalog no.

ELK1466

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Tissue

blood

Standard

16ng/mL

Assay Type

Sandwich

Sensitivity

0.09ng/mL

Detection range

0.25-16ng/mL

Research Area

Hormone metabolism;Bone metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. ELK Biotech supplies this blood related product in 1.

Alternative Names

AQP-CHIP; CHIP28; CO; Channel-Forming Integral Protein,28kDa; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 1, Colton Blood Group (AQP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 1, Colton Blood Group (AQP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 1, Colton Blood Group (AQP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aquaporin 1, Colton Blood Group (AQP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.