2h
Rat aCTx(Alpha-Crosslaps) ELISA Kit
Rat aCTx(Alpha-Crosslaps) ELISA Kit
Assay length
Standard
9000pg/mL
Sensitivity
43.45pg/mL
Research Area
Bone metabolism;
Detection range
111.11-9000pg/mL
Latin name
Rattus norvegicus
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
Alpha CTx，a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction
Description
The aCTx(Alpha-Crosslaps) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Crosslaps (αCTx) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Crosslaps (αCTx) and unlabeled Alpha-Crosslaps (αCTx) (Standards or samples) with the pre-coated antibody specific to Alpha-Crosslaps (αCTx). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Crosslaps (αCTx) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Crosslaps (αCTx) in the sample.