Rabbit vWF(Von Willebrand Factor) ELISA Kit

2h

1000ng/mL

4.64ng/mL

12.35-1000ng/mL

Oryctolagus cuniculus

Competitive Inhibition

Signal transduction;Hematology;

F8VWF; VWD; von Willebrand antigen 2

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample.