3h
Rat HSP90b1(Heat Shock Protein 90kDa Beta 1) ELISA Kit
Rat HSP90b1(Heat Shock Protein 90kDa Beta 1) ELISA Kit
Assay length
Standard
10ng/mL
Assay Type
Sandwich
Sensitivity
0.052ng/mL
Detection range
0.156-10ng/mL
Latin name
Rattus norvegicus
Research Area
Signal transduction;Immune molecule;Developmental science;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Alternative Names
HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1;94 kDa glucose-regulated protein; Endoplasmin
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90β1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90β1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1 (HSP90β1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Beta 1 (HSP90β1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.