Rat HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit

 

Rat HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit

Size

96T

Catalog no.

ELK1614

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

100ng/mL

Assay Type

Sandwich

Sensitivity

0.65ng/mL

Detection range

1.56-100ng/mL

Latin name

Rattus norvegicus

Research Area

Signal transduction;Tumor immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 60kD Protein 1, Chaperonin (HSPD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 60kD Protein 1, Chaperonin (HSPD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.