Rat ANXA5(Annexin A5) ELISA Kit

 

Rat ANXA5(Annexin A5) ELISA Kit

Size

96T

Catalog no.

ELK1649

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.063ng/mL

Detection range

0.156-10ng/mL

Latin name

Rattus norvegicus

Research Area

Apoptosis;Hematology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

ANX5; ENX2; PP4; PAP-I; CBP-I; Anchorin CII; Calphobindin I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; Thromboplastin inhibitor; Vascular anticoagulant-alpha

Description

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. ELK Biotech has more cell death kits and antibodies.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A5 (ANXA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A5 (ANXA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A5 (ANXA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A5 (ANXA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.