Human ProGRP(Pro-Gastrin Releasing Peptide) ELISA Kit

2h

1000pg/mL

4.95pg/mL

12.35-1000pg/mL

Competitive Inhibition

pro-GRP; preproGRP; pre-proGRP

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Tumor immunity;Endocrinology;Reproductive science;Pulmonology;Hepatology;Gastroenterology;Hormone metabolism;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pro-Gastrin Releasing Peptide (ProGRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pro-Gastrin Releasing Peptide (ProGRP) and unlabeled Pro-Gastrin Releasing Peptide (ProGRP) (Standards or samples) with the pre-coated antibody specific to Pro-Gastrin Releasing Peptide (ProGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pro-Gastrin Releasing Peptide (ProGRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pro-Gastrin Releasing Peptide (ProGRP) in the sample.