Rat GSTa1(Glutathione S Transferase Alpha 1) ELISA Kit

3h

20ng/mL

Sandwich

0.133ng/mL

0.312-20ng/mL

Rattus norvegicus

Enzyme & Kinase;Tumor immunity;Hepatology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The GSTa1(Glutathione S Transferase Alpha 1) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

GSTA1; GST2; GSTA1-1; GTH1; GST class-alpha member 1; GST HA subunit 1; GST-epsilon; Glutathione S-transferase A1, N-terminally processed

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 1 (GSTα1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 1 (GSTα1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 1 (GSTα1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Alpha 1 (GSTα1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.