3h
Rat VF(Visfatin) ELISA Kit
Rat VF(Visfatin) ELISA Kit
Assay length
Standard
100ng/mL
Assay Type
Sandwich
Sensitivity
0.61ng/mL
Detection range
1.56-100ng/mL
Latin name
Rattus norvegicus
Research Area
Metabolic pathway;Cardiovascular biology;Hepatology;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.