2h
General OT(Oxytocin) ELISA Kit
General OT(Oxytocin) ELISA Kit
Assay length
Standard
1000pg/mL
Sensitivity
5.51pg/mL
Detection range
12.35-1000pg/mL
Alternative Names
Pitocin; Syntocinon
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
Oxytocin (Oxt) is a hormone, neuropeptide, and medication. The receptor of oxytocin is regulated by an OXTR gene on human chromosome 3p25.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxytocin (OT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxytocin (OT) and unlabeled Oxytocin (OT) (Standards or samples) with the pre-coated antibody specific to Oxytocin (OT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxytocin (OT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxytocin (OT) in the sample.