Human SCT(Secretin) ELISA Kit

2h

1500pg/mL

7.14pg/mL

18.52-1500pg/mL

Competitive Inhibition

Endocrinology;Hepatology;Hormone metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretin (SCT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretin (SCT) and unlabeled Secretin (SCT) (Standards or samples) with the pre-coated antibody specific to Secretin (SCT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretin (SCT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretin (SCT) in the sample.