Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

 

Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

Size

96T

Catalog no.

ELK1843

Price

608 EUR

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Assay length

2h

Standard

200ng/mL

Sensitivity

0.94ng/mL

Alternative Names

NTX-I; NTX1

Detection range

2.47-200ng/mL

Assay Type

Competitive Inhibition

Research Area

Metabolic pathway;Bone metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked N-Telopeptide Of Type I Collagen (NTXI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and unlabeled Cross Linked N-Telopeptide Of Type I Collagen (NTXI) (Standards or samples) with the pre-coated antibody specific to Cross Linked N-Telopeptide Of Type I Collagen (NTXI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in the sample.