2h
General AGE(Advanced Glycation End Product) ELISA Kit
General AGE(Advanced Glycation End Product) ELISA Kit
Assay length
Standard
8000ng/mL
Sensitivity
38.2ng/mL
Detection range
98.8-8000ng/mL
Assay Type
Competitive Inhibition
Alternative Names
AGEs; Advanced Glycation End Products
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Advanced Glycation End Product (AGE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Advanced Glycation End Product (AGE) and unlabeled Advanced Glycation End Product (AGE) (Standards or samples) with the pre-coated antibody specific to Advanced Glycation End Product (AGE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Advanced Glycation End Product (AGE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Advanced Glycation End Product (AGE) in the sample.