Rat COL1a2(Collagen Type I Alpha 2) ELISA Kit

 

Rat COL1a2(Collagen Type I Alpha 2) ELISA Kit

Size

96T

Catalog no.

ELK1986

Price

608 EUR

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Assay length

3h

Standard

100ng/mL

Assay Type

Sandwich

Sensitivity

0.65ng/mL

Detection range

1.56-100ng/mL

Latin name

Rattus norvegicus

Research Area

Metabolic pathway;Bone metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Description

The COL1a2(Collagen I Alpha 2) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1α2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1α2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1α2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 2 (COL1α2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.