2h
Rabbit APOA1(Apolipoprotein A1) ELISA Kit
Rabbit APOA1(Apolipoprotein A1) ELISA Kit
Assay length
Standard
2000ng/mL
Sensitivity
10.53ng/mL
Detection range
24.69-2000ng/mL
Latin name
Oryctolagus cuniculus
Assay Type
Competitive Inhibition
Research Area
Metabolic pathway;Cardiovascular biology;Hepatology;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Apolipoprotein A1 (APOA1) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.