Rat CD14(Cluster Of Differentiation 14) ELISA Kit

 

Rat CD14(Cluster Of Differentiation 14) ELISA Kit

Size

96T

Catalog no.

ELK2116

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Assay Type

Sandwich

Standard

4000pg/mL

Sensitivity

28.4pg/mL

Detection range

62.5-4000pg/mL

Latin name

Rattus norvegicus

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Alternative Names

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 14 (CD14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 14 (CD14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 14 (CD14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 14 (CD14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.