Human S100A9(S100 Calcium Binding Protein A9) ELISA Kit

 

Human S100A9(S100 Calcium Binding Protein A9) ELISA Kit

Size

96T

Catalog no.

ELK2197

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

5.8pg/mL

Assay Type

Sandwich

Standard

1000pg/mL

Detection range

15.6-1000pg/mL

Research Area

Tumor immunity;Infection immunity;Rheumatology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A9 (S100A9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A9 (S100A9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A9 (S100A9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A9 (S100A9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.