3h
Human CD30L(Cluster Of Differentiation 30 Ligand) ELISA Kit
Human CD30L(Cluster Of Differentiation 30 Ligand) ELISA Kit
Assay length
Sensitivity
34pg/mL
Assay Type
Sandwich
Standard
5000pg/mL
Detection range
78-5000pg/mL
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
CD153; TNFSF8; CD30-L; Tumor Necrosis Factor Ligand Superfamily Member 8
Research Area
Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 30 Ligand (CD30L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 30 Ligand (CD30L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 30 Ligand (CD30L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 30 Ligand (CD30L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.