Rabbit COL1a2(Collagen Type I Alpha 2) ELISA Kit

 

Rabbit COL1a2(Collagen Type I Alpha 2) ELISA Kit

Size

96T

Catalog no.

ELK2334

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Standard

20ng/mL

Assay Type

Sandwich

Sensitivity

0.112ng/mL

Detection range

0.312-20ng/mL

Latin name

Oryctolagus cuniculus

Research Area

Metabolic pathway;Bone metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Description

The Rabbit COL1a2(Collagen I Alpha 2) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

About

Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1α2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1α2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1α2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 2 (COL1α2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.