Rat SELP(Selectin, Platelet) ELISA Kit

 

Rat SELP(Selectin, Platelet) ELISA Kit

Size

96T

Catalog no.

ELK2373

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

30pg/mL

Assay Type

Sandwich

Standard

5000pg/mL

Detection range

78-5000pg/mL

Latin name

Rattus norvegicus

Research Area

CD & Adhesion molecule;Tumor immunity;Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

CD62P; P-Selectin; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Description

Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Platelet (SELP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selectin, Platelet (SELP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selectin, Platelet (SELP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selectin, Platelet (SELP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.