Gallus HSPb2(Heat Shock Protein Beta 2) ELISA Kit

3h

5.7pg/mL

Sandwich

1000pg/mL

15.62-1000pg/mL

Signal transduction;Tumor immunity;Developmental science;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

HSP-B2; MKBP; HSP27; Heat Shock 27kDa Protein 2; DMPK-binding protein

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 2 (HSPβ2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 2 (HSPβ2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 2 (HSPβ2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 2 (HSPβ2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.