Rat NAGase(N-Acetyl Beta-D-Glucosaminidase) ELISA Kit

3h

100ng/mL

Sandwich

0.55ng/mL

1.56-100ng/mL

Rattus norvegicus

Enzyme & Kinase;Kidney biomarker;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

N terminal acetylation or CH3CO as epigenetic regulation of NAGase(N- Beta-D-Glucosaminidase) ELISA Kit by NATs.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

MGEA5; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetyl Beta-D-Glucosaminidase (NAGase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acetyl Beta-D-Glucosaminidase (NAGase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acetyl Beta-D-Glucosaminidase (NAGase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acetyl Beta-D-Glucosaminidase (NAGase) in the samples is then determined by comparing the O.D. of the samples to the standard curve.