Rat COL4a1(Collagen Type IV Alpha 1) ELISA Kit

 

Rat COL4a1(Collagen Type IV Alpha 1) ELISA Kit

Size

96T

Catalog no.

ELK2842

Price

608 EUR

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Assay length

3h

Standard

200ng/mL

Assay Type

Sandwich

Sensitivity

1.21ng/mL

Detection range

3.12-200ng/mL

Latin name

Rattus norvegicus

Research Area

Metabolic pathway;

Alternative Names

COL4-A1; Arresten; Collagen Alpha-1(IV)chain

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Description

The COL4a1(Collagen IV Alpha 1) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 1 (COL4α1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV Alpha 1 (COL4α1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 1 (COL4α1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV Alpha 1 (COL4α1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.