Human BCAR1(Breast Cancer Anti-Estrogen Resistance 1) ELISA Kit

3h

cancer

breast

27pg/mL

Sandwich

5000pg/mL

Tumor immunity;

78.12-5000pg/mL

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

CAS; CRKAS; P130Cas; CASS1; Crk-Associated Substrate; Cas Scaffolding Protein Family Member 1

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 1 (BCAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.