3h
Human NET1(Neuroepithelial Cell Transforming Gene 1) ELISA Kit
Human NET1(Neuroepithelial Cell Transforming Gene 1) ELISA Kit
Assay length
Tissue
cell
Standard
20ng/mL
Assay Type
Sandwich
Sensitivity
0.125ng/mL
Detection range
0.312-20ng/mL
Research Area
Neuro science;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
ARHGEF8; NET1A; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8
Description
For cells, cell lines and tissues in culture till half confluency.cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroepithelial Cell Transforming Gene 1 (NET1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroepithelial Cell Transforming Gene 1 (NET1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.