Human TAF13(TATA Box Binding Protein Associated Factor 13) ELISA Kit

3h

20ng/mL

Sandwich

0.128ng/mL

0.312-20ng/mL

Signal transduction;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

TAF2K; TAFII18; Transcription Initiation Factor TFIID Subunit 13; Transcription initiation factor TFIID 18 kDa subunit

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TATA Box Binding Protein Associated Factor 13 (TAF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TATA Box Binding Protein Associated Factor 13 (TAF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.