Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit

 

Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit

Size

96T

Catalog no.

ELK3966

Price

608 EUR

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Assay length

3h

Standard

200ng/mL

Assay Type

Sandwich

Sensitivity

1.35ng/mL

Detection range

3.12-200ng/mL

Latin name

Rattus norvegicus

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F1+2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.