Human LAMP1(Lysosomal Associated Membrane Protein 1) ELISA Kit

 

Human LAMP1(Lysosomal Associated Membrane Protein 1) ELISA Kit

Size

96T

Catalog no.

ELK4001

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.055ng/mL

Detection range

0.156-10ng/mL

Research Area

CD & Adhesion molecule;Tumor immunity;Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

CD107-A; CD107a; LAMPA; LGP120; Lysosome-associated membrane protein 1; CD107 antigen-like family member A

Description

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysosomal Associated Membrane Protein 1 (LAMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysosomal Associated Membrane Protein 1 (LAMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysosomal Associated Membrane Protein 1 (LAMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysosomal Associated Membrane Protein 1 (LAMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.