Human ELA3A(Elastase 3A) ELISA Kit

 

Human ELA3A(Elastase 3A) ELISA Kit

Size

96T

Catalog no.

ELK4101

Price

608 EUR

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Assay length

3h

Assay Type

Sandwich

Standard

2000pg/mL

Sensitivity

13.4pg/mL

Detection range

31.25-2000pg/mL

Research Area

Enzyme & Kinase;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

CELA3A; ELA3; Chymotrypsin-Like Elastase Family Member 3A; Protease E

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3A (ELA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3A (ELA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3A (ELA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3A (ELA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.