Human KISS1R(Kisspeptin Receptor) ELISA Kit

3h

20ng/mL

Sandwich

0.115ng/mL

0.312-20ng/mL

CD & Adhesion molecule;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

AXOR12; GPR54; KiSS1-Derived Peptide Receptor; G Protein-Coupled Receptor 54; Hypogonadotropin-1; Kisspeptins receptor; Metastin receptor

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kisspeptin Receptor (KISS1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kisspeptin Receptor (KISS1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kisspeptin Receptor (KISS1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kisspeptin Receptor (KISS1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.