Cattle IgM(Immunoglobulin M) ELISA Kit

 

Cattle IgM(Immunoglobulin M) ELISA Kit

Size

96T

Catalog no.

ELK4256

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Additional isotype

IgM

Standard

100ng/mL

Assay Type

Sandwich

Sensitivity

0.71ng/mL

Detection range

1.56-100ng/mL

Research Area

Infection immunity;Immune molecule;Hematology;

Alternative Names

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin M (IgM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin M (IgM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin M (IgM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin M (IgM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.