Horse TRF(Transferrin) ELISA Kit

 

Horse TRF(Transferrin) ELISA Kit

Size

96T

Catalog no.

ELK4259

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

34ng/mL

Assay Type

Sandwich

Standard

5000ng/mL

Detection range

78.12-5000ng/mL

Research Area

Tumor immunity;Infection immunity;Immune molecule;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin (TRF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin (TRF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.