3h
Rat L1CAM(L1-Cell Adhesion Molecule) ELISA Kit
Rat L1CAM(L1-Cell Adhesion Molecule) ELISA Kit
Assay length
Tissue
cell
Sensitivity
25pg/mL
Assay Type
Sandwich
Standard
5000pg/mL
Detection range
78.12-5000pg/mL
Latin name
Rattus norvegicus
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
CD & Adhesion molecule;Tumor immunity;Infection immunity;Neuro science;
Alternative Names
CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the L1CAM(L1- Adhesion Molecule) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L1-Cell Adhesion Molecule (L1CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L1-Cell Adhesion Molecule (L1CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L1-Cell Adhesion Molecule (L1CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L1-Cell Adhesion Molecule (L1CAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.