Rat PKCe(Protein Kinase C Epsilon) ELISA Kit

 

Rat PKCe(Protein Kinase C Epsilon) ELISA Kit

Size

96T

Catalog no.

ELK4533

Price

608 EUR

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Assay length

3h

Standard

100ng/mL

Assay Type

Sandwich

Sensitivity

0.58ng/mL

Detection range

1.56-100ng/mL

Latin name

Rattus norvegicus

Alternative Names

PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Epsilon (PKCe), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Epsilon (PKCe) in the samples is then determined by comparing the O.D. of the samples to the standard curve.