Rat PSGL1(P-Selectin Glycoprotein Ligand 1) ELISA Kit

3h

Sandwich

4000pg/mL

28.5pg/mL

62.50-4000pg/mL

Rattus norvegicus

CD162; SELPLG; CLA; Selectin P Ligand

CD & Adhesion molecule;Tumor immunity;Infection immunity;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin Glycoprotein Ligand 1 (PSGL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to P-Selectin Glycoprotein Ligand 1 (PSGL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P-Selectin Glycoprotein Ligand 1 (PSGL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of P-Selectin Glycoprotein Ligand 1 (PSGL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.