Rat HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit

3h

cell

10ng/mL

Sandwich

0.062ng/mL

0.156-10ng/mL

Rattus norvegicus

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Hematology;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the HCAM(Homing Associated Adhesion Molecule) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homing Associated Cell Adhesion Molecule (HCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homing Associated Cell Adhesion Molecule (HCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.