Human UBA52(Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) ELISA Kit

3h

20ng/mL

Sandwich

0.116ng/mL

0.312-20ng/mL

Signal transduction;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

CEP52; HUBCEP52; RPL40; 60S Ribosomal Protein L40; Ubiquitin Carboxyl Extension Protein 52

Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) in the samples is then determined by comparing the O.D. of the samples to the standard curve.