Rat IgG1(Immunoglobulin G1) ELISA Kit

2h

4000ng/mL

18.7ng/mL

49.4-4000ng/mL

Immune molecule;

Rattus norvegicus

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G1 (IgG1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G1 (IgG1) and unlabeled Immunoglobulin G1 (IgG1) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G1 (IgG1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G1 (IgG1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G1 (IgG1) in the sample.