2h
96T
ELK4853
608 EUR
2h
5000pg/mL
25.3pg/mL
61.7-5000pg/mL
Bone metabolism;
Competitive Inhibition
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alpha CTx,a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction
The aCTx(Alpha-Crosslaps) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Crosslaps (αCTx) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Crosslaps (αCTx) and unlabeled Alpha-Crosslaps (αCTx) (Standards or samples) with the pre-coated antibody specific to Alpha-Crosslaps (αCTx). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Crosslaps (αCTx) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Crosslaps (αCTx) in the sample.