Rat NES1(Nesfatin 1) ELISA Kit

2h

40000pg/mL

182.5pg/mL

493.8-40000pg/mL

Rattus norvegicus

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nesfatin 1 (NES1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nesfatin 1 (NES1) and unlabeled Nesfatin 1 (NES1) (Standards or samples) with the pre-coated antibody specific to Nesfatin 1 (NES1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nesfatin 1 (NES1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nesfatin 1 (NES1) in the sample.