Human DDR2(Discoidin Domain Receptor Family, Member 2) ELISA Kit

 

Human DDR2(Discoidin Domain Receptor Family, Member 2) ELISA Kit

Size

96T

Catalog no.

ELK5000

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

100ng/mL

Assay Type

Sandwich

Sensitivity

0.54ng/mL

Detection range

1.56-100ng/mL

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Signal transduction;CD & Adhesion molecule;Developmental science;

Alternative Names

CD167b; MIG20a; NTRKR3; TKT; TYRO10; Discoidin Domain Receptor Tyrosine Kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discoidin Domain Receptor Family, Member 2 (DDR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discoidin Domain Receptor Family, Member 2 (DDR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discoidin Domain Receptor Family, Member 2 (DDR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Discoidin Domain Receptor Family, Member 2 (DDR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.