2h
Rat FGF7(Fibroblast Growth Factor 7) ELISA Kit
Rat FGF7(Fibroblast Growth Factor 7) ELISA Kit
Assay length
Standard
1000pg/mL
Sensitivity
5.37pg/mL
Detection range
12.35-1000pg/mL
Latin name
Rattus norvegicus
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7
Research Area
Cytokine;Tumor immunity;Infection immunity;Developmental science;Pulmonology;Dermatology;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 7 (FGF7) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 7 (FGF7) and unlabeled Fibroblast Growth Factor 7 (FGF7) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 7 (FGF7). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 7 (FGF7) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 7 (FGF7) in the sample.