3h
Rat a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
Rat a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
Assay length
Standard
100ng/mL
Assay Type
Sandwich
Sensitivity
0.69ng/mL
Detection range
1.56-100ng/mL
Latin name
Rattus norvegicus
Research Area
Infection immunity;Immune molecule;Kidney biomarker;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Alternative Names
AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
The a1M(Alpha-1-Microglobulin/Bikunin ) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.Precursor cell, also called a blast cell or simply blast, is a type of partially differentiated, usually unipotent cell that has lost most or all of the stem cell multipotency.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (α1M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (α1M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Microglobulin/Bikunin Precursor (α1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor (α1M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.