Rabbit SAA(Serum Amyloid A) ELISA Kit

2h

Sera

serum

9ng/mL

400ng/mL

25-400ng/mL

Oryctolagus cuniculus

Competitive Inhibition

Infection immunity;Kidney biomarker;Hepatology;Urology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Serum Amyloid A (SAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Serum Amyloid A (SAA) and unlabeled Serum Amyloid A (SAA) (Standards or samples) with the pre-coated antibody specific to Serum Amyloid A (SAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Serum Amyloid A (SAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Serum Amyloid A (SAA) in the sample.